kapa hyperplus library preparation kit (Roche)

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Roche kapa hyperplus library preparation kit
Kapa Hyperplus Library Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kapa+hyperplus+library+preparation+kit/HyperPrep/10__1158_slash_1078___0432__ccr___25___2743-274-10-15
Average 99 stars, based on 5 article reviews

kapa hyperplus library preparation kit - by Bioz Stars, 2026-09

99/100 stars

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Polymerase Chain Reaction:

Article Title: Antibodies that bind adenosine A2A receptors and methods of use thereof to treat cancer and neurological diseases
Article Snippet: .. The PCR product was directly used for library preparation using the KAPA HyperPlus Library Preparation Kit (Kapa Biosystems, product #KK8514). .. To add diversity in the library, the samples were spiked with 15% PhiX Control purchased from Illumina, Inc. (product #FC-110-3001).

Article Title: Polymerase trapping as the mechanism of H5 highly pathogenic avian influenza virus genesis.
Article Snippet: INTRODUCTION: Influenza viruses pose a continuous health threat to animals and humans worldwide.. Outbreaks of highly pathogenic avian influenza viruses (HPAIVs) have a devastating impact on domestic and wild bird populations as well as on wild mammals.. Moreover, HPAIVs present a health risk to humans through repeated spillover events, renewing concerns about the potential for a new pandemic.

Construct:

Article Title: Unveiling a hidden threat: whole genome sequencing exposes the cross-hospital spread of extensively drug-resistant Pseudomonas aeruginosa
Article Snippet: .. Briefly, DNA was extracted using the DNeasy UltraClean microbial kit (Qiagen, Germantown, MD, United States), and libraries were constructed using the Kapa HyperPlus library preparation kit (Roche Diagnostics, Indianapolis, IN, United States). .. Libraries were quantified using the Kapa library quantification kit Illumina/Bio-Rad iCycler (Roche Diagnostics) on a CFX96 real-time cycler (Bio-Rad, Hercules, CA, United States).

Article Title: Characterizing the nasopharyngeal microbiome and resistome of dairy cattle with and without bovine respiratory disease.
Article Snippet: DNA was analyzed on the Agilent 2200 TapeStation System using the Genomic DNA ScreenTape assay for the integrity of gDNA. .. Libraries were constructed using the KAPA HyperPlus Library Preparation Kit (Roche, Indianapolis, Indiana, USA). .. Shotgun sequencing was performed using the Illumina NovaSeq 6000 platform with an S4 flow cell (2 × 150 bp) (Illumina Inc., San Diego, California, USA).

Selection:

Library Amplification:

Purification:

Article Title: Parallel analysis of replication timing, gene expression, and copy number with PARTAGE.
Article Snippet: .. Briefly, purified DNA from sorted cells was directly processed using the KAPA HyperPlus library preparation kit (Roche, #07958978001) according to the manufacturer’s instructions. .. Enzymatic fragmentation was performed at 37°C for 30min, followed by end-repair and ligation of KAPA dual-indexed adapters (Roche, #08278555702).

Generated:

Article Title: Assessment of pathogenic potential in non-pathogenic industrially relevant bacteria
Article Snippet: .. Genomic libraries were generated for most of the strains using modified Kapa HyperPlus Library Preparation Kit (Roche, Switzerland) on Biomek i5 Liquid Handler (Beckman Coulter, USA). ..

Modification:

Sequencing:

Article Title: Use of Metagenomic Reference Materials to Compare Lysis and Extraction Methods Against a Known Input
Article Snippet: Prior to library preparation, DNA was also quantified via Qubit dsDNA HS Assay Kit (Thermo Fisher), or the Qubit dsDNA BR Assay Kit (Thermo Fisher) in instances when the sample was out of range of the HS kit. .. Sequencing libraries were prepared from microbial DNA using the KAPA HyperPlus Library Preparation Kit (Kapa Biosystems, Wilmington, MA) with up to 100 ng DNA input following the manufacturer’s protocol using internal single-index 8 bp barcoding sequences and TruSeq Adapters (Illumina, San Diego, CA). .. The DNA purified from the ZymoBIOMICS Microbial Community Standard was quantified using QuantiFluor® ONE fluorescent dye, and samples were diluted to a final concentration of 1ng/μl prior to sequencing.

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